FIGURE 6.

Uptake of dsRNA synthesized in vitro by hyphae of Rhizoctonia solani and the target gene silenced. (a) Quantitative gene expression pattern of Rstps2 after supplementation of the dsRNA. The data were normalized to the GAPDH transcript levels of GD‐118 and are shown as the mean ± SE of triplicates. Different labels represent statistical significance (p < .05), one‐way analysis of variance and Tukey's test. (b) Sclerotial differentiation (SDIF) of sclerotia of R. solani AG‐1 IA strain GD‐118 treated with dsRNA for 10 days. Data are shown as the mean ± SE of triplicates. *p < .05, Student's t test. (c) Microscopic observation of the uptake of dsRNA with fluorescently labelled dsRNA and without fluorescently labelled dsRNA of Rstps2 by GD‐118 cells