TABLE 3.
Primers used in this study
| Primer name | Sequence | Use in this study |
|---|---|---|
| CgMsb2‐5Ffor (1F) | TCAACTGAGGCGGTTATTCC | 5F flanking sequence |
| CgMsb2‐5Frev (2R) | GAGCGACTCGACGAGAAATC | |
| CgMsb2‐3Ffor (2F) | TACGCTTCGATACCCTTCGG | 3F flanking sequence |
| CgMsb2‐3Frev (3R) | CTTGGAGCGACAAGTTGGGA | |
| External‐CgMsb2for | TTCCTAGCGACCCTGTTGTT | External sequence used for validation of mutant |
| External‐CgMsb2rev | GCCCGGTGAGTAGAATGGTA | |
| Internal‐CgMsb2for | GCTATTGGAATCGGTGCTGT | Internal sequence used for validation of mutant |
| Internal‐CgMsb2rev | GGCGACTCCACCGTAGTTAG | |
| CgMsb2‐Compfor | TCAACTGAGGCGGTTATTCC | Complementary sequence |
| CgMsb2‐Comprev | GCCCGGTGAGTAGAATGGTA | |
| Hygromycinfor (HYG‐F) | CGCCAGGGTTTTCCCAGTCACGAC | Hygromycin cassette contains the region of M13F and M13R |
| Hygromycinrev (HYG‐R) | AGCGGATAACAATTTCACACAGGA | |
| YG‐F | GATGTAGGAGGGCGTGGATATGTCCT | The second/third portion of the hygromycin cassette |
| HY‐R | GATGTAGGAGGGCGTGGATATGTCCT | |
| SUR‐5′‐M13F | CGCCAGGGTTTTCCCAGTCACGACGTCGACGTGCCAACGCCACAG | Sur cassette contains the region of M13F and M13R |
| SUR‐3′‐M13R | AGCGGATAACAATTTCACACAGGAGTCGACGTGAGAGCATGCAAT | |
| SU‐SPLIT | CCAAGCATGTGCAGTGCCTTC | The second/third portion of the Sur cassette |
| UR‐SPLIT | GGAGGCCGACGTCATAGGCATC | |
| CgSho1‐5Ffor (1F) | AGTCTCCCACCTTCACTGTG | 5F flanking sequence |
| CgSho1‐5Frev (2R) | GTAGAATTGGCGAGGCAGTG | |
| CgSho1‐3Ffor (2F) | GATGGTTTTCGCGCTCAATG | 3F flanking sequence |
| CgSho1‐3Frev (3R) | GCGCAACATTCCTCACTCAA | |
| External‐CgSho1for | CACCACATTGCTGCTGTCTT | External sequence used for validation of mutant |
| External‐CgSho1rev | CTCTTGATGTTGCGAGGCTC | |
| Internal‐CgSho1for | TGGATCTTCTACTTCGGCTCA | Internal sequence used for validation of mutant |
| Internal‐CgSho1rev | GCGCCGAAGTGTACATTTGA | |
| CgSho1‐Compfor | AGTCTCCCACCTTCACTGTG | Complementary sequence |
| CgSho1‐Comprev | TAACAGGATCAGGTAGTTGC | |
| CgPth11‐5Ffor (1F) | TGGTCAAGTCTGTGGGTTGT | 5F flanking sequence |
| CgPth11‐5Frev (2R) | CAAGAAAAGCCGCTGACCAT | |
| CgPth11‐3Ffor (2F) | ATATCCCTGGCTTTGGTCGG | 3F flanking sequence |
| CgPth11‐3Frev (3R) | CAAGCCATGCCTTCCAGATC | |
| External‐CgPth11for | ATTCAACCGCCTCTTCTGGA | External sequence used for validation of mutant |
| External‐CgPth11rev | TATGTCACTCGATCTGGGGC | |
| Internal‐CgPth11 for | TTCTCAGCAACCTACGACGT | Internal sequence used for validation of mutant |
| Internal‐CgPth11rev | AAATCGATCGTCTCAGCGGA | |
| CgPth11‐Compfor | ATTCAACCGCCTCTTCTGGA | Complementary sequence |
| CgPth11‐Comprev | CCGACCAAAGCCAGGGATAT | |
| CgSte7S218E T222E for | AGAACTTATCAACGAGGTTGCAGACGAATTCGTTGGAACAT | Constitutively active allele of Ste7 |
| CgSte7S218E T222E rev | ATGTTCCAACGAATTCGTCTGCAACCTCGTTGATAAGTTCT |