FIGURE 3.
Changes in NADPH oxidase activity, H2O2 content and transcript levels of PpMAPKKK5s/PpMAPKK5, salicylic acid (SA) biosynthesis, and SA‐responsive genes in peaches after inoculation with Rhizopus stolonifer at 12 hr intervals. NADPH oxidase activity (a) and H2O2 content (b) were recorded as the mean ± SE. Asterisks indicate significant (p = 0.05 level) differences among BABA‐treated, R. stolonifer‐inoculated, and untreated peaches. mRNA expression levels, including PpMAPKKK5s/PpMAPKK5 (c) and SA biosynthesis and SA‐responsive genes (d), were profiled using quantitative reverse transcription PCR and visualized with heatmap. Peach TEF2 was used as an internal standard. Data represent means from six measurements