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. 2021 Sep 8;22(12):1624–1640. doi: 10.1111/mpp.13134

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© 2021 The Authors. Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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CRISPR/Cas9‐mediated MAPKK5 knockout lines showed slightly decreased resistance to the fungal pathogen Rhizopus stolonifer. (a) AtMAPKK5 transcription in 4‐week‐old wild‐type (WT) and knockout lines. (b, c) MAPKK5 knockout did not change the speed of growth of Arabidopsis. (d) Transcription of NPR1 and PRs in WTs and knockout plants (CRP3, CRP7, and CRP11) after inoculation with R. stolonifer at 3 and 6 days postinoculation (dpi). (e) Disease symptoms of WTs and knockout plants at 6 dpi. (f) Necrotic areas in WTs and knockout plants were assessed by trypan blue staining at 6 dpi. (g) Electrolyte leakage levels of WTs and knockout plants infected with R. stolonifer at 6 dpi