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Analysis of ENM-bound proteins. 800 μg of MWCNT-7, TiO2, SiO2, or C60 was incubated with 1 ml of 20-fold concentrated rat BALF at 37°C for 4-6 hr. The bound proteins were then disassociated from the ENMs and analyzed by SDS-PAGE and LC/MS. (A) imagine of SDS-PAGE; (B) Venn diagram showing the distribution of the proteins bound to the 4 ENMs; and (C) abundance of SP-A, SP-D, and SP-B bound to each type of ENM.
