Figure 6.

Effect of CD14 knockdown on phagocytosis of MWCNT-7, TiO₂, and SiO₂. Polarized light microscope images of primary alveolar macrophages treated with MWCNT-7 pre-incubated with SP-A (A); CD14-knockdown primary alveolar macrophages treated MWCNT-7 pre-incubated with SP-A (B); primary alveolar macrophages treated with TiO₂ pre-incubated with SP-A (C); CD14-knockdown primary alveolar macrophages treated with TiO₂ pre-incubated with SP-A (D); primary alveolar macrophages treated with TiO₂ pre-incubated with SP-D (E); CD14-knockdown primary alveolar macrophages treated with TiO₂ pre-incubated with SP-A (F); primary alveolar macrophages treated with SiO₂ pre-incubated with SP-A (G); CD14-knockdown primary alveolar macrophages treated with SiO₂ pre-incubated with SP-A (H); primary alveolar macrophages treated with SiO₂ pre-incubated with SP-D (I); and CD14-knockdown primary alveolar macrophages treated with SiO₂ pre-incubated with SP-D (J). Percentages of alveolar macrophages with phagocytosed MWCNT-7, TiO₂, and SiO₂ were shown (K). Arrows indicate alveolar macrophages with phagocytosed ENMs; bars = 50 μm. *** and **** represent p values less than 0.001 and 0.0001, respectively.