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. 2000 Jun;20(12):4199–4209. doi: 10.1128/mcb.20.12.4199-4209.2000

FIG. 8.

FIG. 8

The Ste12p C terminus can cause activation by multimerization in vivo. (A) Yeast strain YCN7 (ste12 dig1 dig2) bearing pSH18-34 (LexA ops-LacZ) was transformed with pMHLex expressing LexA (vector) or the LexA-Ste12p expression plasmids pIS182 (216 to 688), pIS184 (216 to 500), pIS183 (216 to 474), pIS187 (262 to 688), pIS188 (356 to 688), pIS189 (403 to 688), and pIS194 (450 to 688). Transcriptional activation by LexA fusions was assayed by measuring β-galactosidase (β-Gal) activity in cells grown to mid-log phase. (B) Yeast strain YCN7 bearing pSH18-34 and the LexA-Ste12p bait plasmids (as in panel A; LexA-Ste12p Bait) was cotransformed with vectors producing residues 216 to 288, 356 to 688, or 216 to 500 of Ste12p from a GAL promoter (Ste12p Prey). Cells were grown to mid-log phase, and expression from the LexA-responsive reporter was measured by assaying β-galactosidase activity 2 h after galactose addition.