TABLE 2.
Oligonucleotides
| Name | Ste12p amino acida | Sequence (5′ to 3′) |
|---|---|---|
| AO1 | 215F | GGTACCATGTCTAGAAGACCATCTAGTACAACA |
| AO7 | 262F | GGTACCATGCCCTCTCAAATTAATGATTTTATT |
| AO8 | 309F | GGTACCATGGACTATTTTCTTGTATCTGTTGAA |
| AO9 | 351F | GGTACCATGTCTCTTCTTAATAGATACCCCTAT |
| AO10 | 403F | GGTACCATGGACCCTACCAGCTACATGAAGTAT |
| AO11 | 450F | GGTACCATGCAATCTTACCCAAACGGAATGGTT |
| AO12 | 500R | GAATTCTCATTGTGGATACAGCATATTGTTATC |
| AO13 | 547R | GAATTCTCACTGCATGGAATTTGAACTTTGCAT |
| AO14 | 594R | GAATTCTCAATTTCCTTGTGAAGACTTCATTCC |
| AO15 | 641R | GAATTCTCAAGAATCTTCGTCACCAGCACTTGG |
| AO4 | 688R | GAATTCTCAGGTTGCATCTGGAAGGTTTTTATC |
| VT1 | 1F | CTGGATCCATGAAAGTCCAAATAACCAATAGT |
| SBD1 | 21F | CTGGATCCATGGAAAACGATGAAGTCAGTAAAGCT |
| AO29 | 45F | CTGGATCCATGTTCTTTTTAGCCACAGCG |
| VT2 | 215R | TCGAATTCTCATCTAGAATCTAAATGTTGAAGTAA |
| SDB2 | 195R | TCGAATTCTCATGAAAAAGATAAGGCGGGCTCATT |
| AO27 | 170R | TCGAATTCTCATTCCAACGCATCCG |
a
Terminal Ste12p amino acid residue encoded by amplified DNA fragments produced with the indicated oligonucleotide. Priming direction: F, forward; R, reverse.