Figure 1.

Chemotherapeutic treatment promotes HMGB1 expression and induces HMGB1 translocation. (A) Effect of DOX on the proliferation of BEL7402, BEL7402/DOX, SMMC7721, and SMMC7721/DOX cells, and DOX IC₅₀ in these cells. The IC₅₀ values of BEL7402/DOX and SMMC7721/DOX cells were higher than those of the parental cells, **P < 0.01. (B) Treatment with DOX increased the expression of HMGB1 and promoted HMGB1 translocation in a dose- and time-dependent manner. (C) Lysates of parental and DOX-resistant HCC cells were prepared to detect HMGB1. Total and cytoplasmic expression of HMGB1 were higher in DOX-resistant HCC cells.