Figure 2.

Inhibition of HMGB1 expression and cytoplasmic translocation increases sensitivity to DOX in HCC cells. (A) Compared with Si-NC cells, the level of HMGB1 was significantly decreased in Si-HMGB1 cells. (B) Suppression of HMGB1 expression and cytoplasmic translocation in HCC cells decreased the IC₅₀ value of DOX. **P < 0.01 compared with Si-NC cells. (C) Inhibition of HMGB1 expression and cytoplasmic translocation enhanced apoptosis sensitivity of HCC cells to DOX. Apoptosis was analyzed by flow cytometric analysis of annexin-V/PI staining. **P < 0.01 compared with Si-NC cells.