FIGURE 5.

Effect of the expression of EptA variants mutated in a catalytic residue (EptA^T278A) or deleted of 22 amino acids at the C-terminus (EptA^ΔC–ter) in P. aeruginosa. (A) MALDI-TOF spectra of lipid A extracted from PAO1 or PA14 containing pMEeptA, pMEeptA^T278A, or pMEeptA^ΔC–ter cultured in the presence of 0.125 mM IPTG. The m/z values of peaks corresponding to phosphoethanolaminated lipid A forms are highlighted in yellow. See the legend to Figure 1 for further details. (B) Growth curves of PAO1 or PA14 containing pMEeptA, pMEeptA^T278A, or pMEeptA^ΔC–ter cultured in the presence of the indicated IPTG concentrations. Data represent the mean (±SD) or are representative of at least three independent experiments.