TABLE 2.

Cell cycle distribution of HIp53 cells after ADR treatment

DNA content or cell cycle phase	Time after ADR treatment (h)	% of cells after treatment with ADRa at:
		90 nM		175 nM		350 nM		525 nM
		−	+	−	+	−	+	−	+
<2 N	17	0		0		0		0
G1	17	50		29		28		19
S	17	19		20		19		39
G2/M	17	31		51		53		42
<2 N	24	0	0	0	0	0	0	0	1
G1	24	70	71	50	51	13	23	7	10
S	24	11	8	7	5	8	5	6	4
G2/M	24	19	21	43	44	79	72	87	85
<2 N	48	0	0	11	4	13	3	5	2
G1	48	44	73	34	50	18	15	4	11
S	48	17	6	15	6	16	7	7	4
G2/M	48	39	21	40	40	53b	75	84b	83
<2 N	72	0	0	5		27	2	9	4
G1	72	65	71	54	53	28	17	9	11
S	72	11	7	12	7	18	6	8	4
G2/M	72	24	22	29	40	27b	75	74b	81

^aResults are representative of two independent experiments. Values for a control, untreated culture were as follows: <2 N, 0%; G₁, 71%; S, 12%; G₂/M, 17%. − and +, absence and presence of PonA (plus p53), respectively. 

^bMore than 75% of the cells underwent cell death and detached from the plate; the number is representative of the remaining cells (see Fig. 2B).