Skip to main content
. 2021 Oct 27;12:751667. doi: 10.3389/fphar.2021.751667

FIGURE 2.

FIGURE 2

CircHIPK3 interacted with miR-149-5p, and FOXO1 was a target of miR-149-5p. (A) MiR-149-5p was pulled down and enriched with biotinylated circHIPK3 probe (n = 3; **p < 0.01). (B) The relative luciferase activity of circHIPK3 was markedly suppressed when co-transfected with wild circHIPK3 and miR-149-5p mimics compared with the control reporter or mutated luciferase reporter (n = 3; ***p < 0.001). (C) qRT–PCR detection showed that the expression of miR-149-5p was significantly increased/reduced in the circHIPK3 knockdown/overexpression group compared with each control group (n = 3; *p < 0.05, **p < 0.01). (D) The level of circHIPK3 could be significantly reduced/enhanced by miR-149-5p mimics/inhibitor compared with each control group (n = 3; *p < 0.05, **p < 0.01). (E) Luciferase reporter assays showed that the luciferase activity of FOXO1 wild-type reporter was significantly reduced when transfected with miR-149-5p mimics compared with control reporter or mutated luciferase reporter (n = 3; ***p < 0.001). (F) A significant enrichment of FOXO1 mRNA in the pulled down sediments of 3′ end biotin-labeled miR-149-5p. (n = 3; **p < 0.01). Results are expressed as the mean ± S.E.M. CTL = PBS control group. si-scrl = scramble siRNA. NC = negative control.