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. 2021 Oct 27;9:758561. doi: 10.3389/fbioe.2021.758561

FIGURE 1.

FIGURE 1

Internal promoters in the epothilone operon and their promotion activities. (A) Prediction of internal promoters in the epothilone operon from S. cellulosum So0157-2. The promoter prediction was performed using two online promoter prediction programs: “Neural Network Promoter Prediction” (red arrows) and BPROM (green arrows). (B) RT-qPCR analysis of expression levels of the seven operon genes in ZE9 after 48 h of incubation. The epoA expression was set as 1, and the expressions of the other six genes are shown as the relative expression of the epoA gene. (C) Activities of the separate internal promoters in E. coli. The activity of the reporter gene chloramphenicol acetyltransferase (CAT) was detected by using the CAT ELISA kit, with the aphII promoter as a positive control and the original plasmid pKK-232 as a negative control (no promoter upstream of the reporter gene CAT).