TABLE 1.

Information of the spacer sequences.

Name	Spacer	Coding strand	Off-target	Distance (bp)	Δg (kcals/mol)	Hairpin	GC%
CuOm-P	TCC​GGG​GGA​TGA​TGC​TCG​AG	+	8	−617	43.6	N	65.0
CuOm-B	TGA​GGA​GCC​TGT​TGC​AGA​AG	+	14	−376	38.2	N	55.0
CuOm-C	ACC​GTA​CCG​GCA​ACG​CTG​TTG	+	4	−165	45.3	N	61.9
CuOm-D	TGC​GGC​CGG​TAT​CCT​GGA​CGA	+	3	−117	47.8	N	66.7
CuOm-E	TGG​ATG​TAT​CCC​AAG​GTG​CT	+	2	−160	38.1	N	50.0
CuOm-F	AGC​TCT​TCT​TCC​GAA​ATG​CCG	+	2	−193	43.5	N	52.4