Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Sequences of primers and probes used in the qPCR reactions as well as multiple parameters calculated during these developments. Hairpin and dimer values are expressed in standard Gibbs free energy (ΔG kcal/mole). Designs were carried out using the RealTime qPCR Assay tool. Probes were labeled at 5′ with FAM (Fluorescein amidites) or VIC (Victoria) dyes and at 3′ with MGB-NFQ (Minor groove binder-non fluorescent quencher).
