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. 2021 Oct 27;12:769965. doi: 10.3389/fmicb.2021.769965

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Copyright © 2021 Moro, Wu, Wei, Mendes, Allen, Pinheiro, Clough and Zucchi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Mapping of collection sites of E. heros and P. guildinii, classified by ecoregion in Brazil. (B) NMDS of Bray-Curtis similarity matrix among 115 samples from the intestinal microbiome of E. heros and P. guildinii, for the different ecoregion from Brazil. The ellipses are showing clustering between ecoregion. Multivariate analysis of variance (PERMANOVA) is indicated in the upper right. (C) Alpha diversity levels based on Observed OTU, Chao1 index and Shannon index for the different ecoregion. The non-parametric test (Kruskal–Wallis) is indicated in the upper left of each graph. Pairwise comparison (Wilcoxon) is indicated between Ecoregions. (D) Analysis of the ten most abundant bacteria families in the intestinal microbiome of E. heros and P. guildinii collected in Brazil, classified by ecoregion.