Skip to main content
. 2021 Oct 27;11:740704. doi: 10.3389/fcimb.2021.740704

Figure 5.

Figure 5

COX-2 induction, PGE2 release, and cell proliferation by SGS. (A) Expression of COX-2 in HT29 cells was examined by qPCR after exposure of these cells to S. gallolyticus UCN34 at an MOI of 20 for 4 h COX-2 was significantly upregulated as determined by one-way ANOVA (*p < 0.05). (B) PGE2 release after exposure to secretomes of S. gallolyticus subsp. gallolyticus (UCN34/NTB12), S. gallolyticus subsp. pasteurianus (NTB7/992), S. gallolyticus subsp. macedonicus (19AS/ACA-DC-205), and S. equinus/lutetiensis (NCTC8133/NTB2). No significant increase in PGE2 release was observed after 24 h. (C) Cell growth was measured with MTT assay at 24, 48, and 72 h in HT29, Caco-2, HCT116, and SW480 cells. Each secretome condition was performed in quadruplicate. The area under the curve was calculated for each condition and compared to control cells using independent t-test. HCT116 cells were most sensitive to S. bovis group bacteria. In none of the conditions, S. gallolyticus subsp. gallolyticus secretomes induced cell growth. S. gallolyticus subsp. gallolyticus NTB12 even inhibited cell growth in HCT116 cells. Only S. gallolyticus subsp. pastereurianus strains 992 and NTB7 were able to induce cell growth in HCT116 cells. S. lutetiensis NTB2 consistently inhibited cell growth in CaCo-2, SW480, and HCT116 cells. *p < 0.05, **p < 0.01, ***p < 0.001.