TABLE 1.

Oligonucleotide primers used for the interference assays and relative incorporation of a single [³²P]dGMP residue into oligos tel1 through tel8

Oligo	Sequencea	No. of nucleotides	Relative incorporationb ± SD
tel1	TTGGGGTGCTTGTAGGTTGGG	21	100 ± 9
tel2	TTGGGGTGCTCGAGGTAGGG	20	5.3 ± 2
tel3	TTGGGGTGCTCGAGGATGGG	20	49 ± 6
tel4	TTGGGGTGCTTGTAGATTGGG	21	93 ± 32
tel5	TTGGGGTGCTTGTAGGTTTTGGG	23	173 ± 54
tel6	TTGGGGTTGGGGTTGGG	17	29 ± 1.7
tel7	TAGTCGTGCTTGTACATTGGG	20	118 ± 11
tel8	TTCCGTTAGTCGAGTTGGGGCGCTTGCAGGTTGGG	35	104 ± 12
tel9	TTGGGGTGCTTGTAGGTTGGGGT	23

^aTelomeric repeats are indicated by bold letters. Substitutions and insertions are underlined. 

^bThe incorporation of a single [³²P]dGMP residue into oligos tel1 to tel8 was determined in assays of the type shown in Fig. 2B. A labeled oligonucleotide was added to each sample before the ethanol precipitation step, and the radioactivity recovered in this oligonucleotide was used to correct the data for losses that occurred during the procedure. The data are expressed relative to the incorporation into oligo tel1, which was set at 100. Each assay was repeated three times. Average values and standard deviations are shown here.