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. 2021 Nov 8;221(1):e202106100. doi: 10.1083/jcb.202106100

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© 2021 Pennauer et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).

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Figure 3. — Analysis of the ultrastructure of the Golgi. (A) Thin section transmission electron microscopy was performed to examine the ultrastructure of cellular compartments in all Arf KO cell lines. The field of view was chosen to include Golgi stacks in the acquired images. G, Golgi stack; arrowheads indicate dilated ER; scale bar, 500 nm. (B–D) Individual Golgi stacks were quantified for their apparent length (B), number of cisternae per stack (C), and thickness (D) in electron microscopy images of HeLaα and Arf1ko cells. Approximately 40 Golgi stacks were quantified per cell line. Bars, mean ± SD. Unpaired, two-tailed t test (ns, P > 0.05; ***, P < 0.001).