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. 2021 Nov 8;221(1):e202106100. doi: 10.1083/jcb.202106100

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© 2021 Pennauer et al.

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Figure S5. — Intracellular localization and protein level of KDEL receptors. (A) Parental HeLaα, Arf1ko, and Arf4ko cells were coimmunostained for KDEL receptors (KDELRs) in green and GM130 in magenta. DAPI signal is shown in blue. Lower panels show magnified image sections. Scale bars, 10 µm. (B) Perinuclear localization of the KDEL receptors was quantified as a ratio to GM130. Average values obtained from three independent experiments with >40 Golgi analyzed per cell line and experiment are shown as filled circles together with the values from individual cells as small dots colored by experiment. Mean ± SD of the three experiments are indicated. Unpaired one-way ANOVA versus parental (***, P < 0.001). (C) KDEL receptor levels in parental HeLaα and Arf KO cell lines were analyzed by immunoblot analysis with actin as a loading control. For each cell line, three biological replicates were analyzed on the same gels with parental lysates.