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. 2021 Nov 8;221(1):e202103156. doi: 10.1083/jcb.202103156

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© 2021 Zheng et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S2. — The effect of knocking down PEX19. (A) Representative images of WT HeLa cells expressing MARCH5-mCherry and Ub-GFP-SKL. Endogenous PEX19 was knocked down by using shRNA. (B) Percentage of MARCH5-mCherry colocalized with peroxisome from A was calculated in ImageJ using Mander’s colocalization coefficients. More than 50 cells were counted. Error bars indicate SD. **, P < 0.01; n.s., not significant (one-way ANOVA and Tukey’s multiple comparisons test). (C) The knockdown effect of PEX19 shRNAs was verified at the protein level. (D) RFP-GFP-SKL HeLa cells transfected with two different shRNAs targeting PEX19 were treated with Torin1 (1 μM for 24 h), folimycin (10 nM for 24 h), or a combination of the two. Cells were then analyzed by flow cytometry.