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. 2021 Nov 8;221(1):e202103156. doi: 10.1083/jcb.202103156

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© 2021 Zheng et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

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Figure S3. — MARCH5 ubiquitinates PEX3. (A) HeLa cells were cotransfected with MARCH5-Myc and PEX3-V5 plasmids for 18 h and treated with 10 μM MG132 for 6 h in the indicated sample. IP and IBs were performed with anti-Myc and anti-V5 antibodies. (B) MARCH5 KO HeLa cells were cotransfected with HA-Ub, PEX3-3×FLAG, and MARCH5-Myc for 18 h and treated with MG132 (10 μM) for 6 h. IP was performed with anti-FLAG antibody and followed by IB with indicated antibodies. (C) 293FT cells were cotransfected with HA-Ub, PEX3-3×FLAG, and MARCH5-Myc for 48 h. Cells were treated with DMSO, MG132 (10 μM for 6 h), or folimycin (10 nM for 24 h). IP was performed with anti-FLAG antibody and followed by IB with indicated antibodies. L.E., long exposure; S.E., short exposure.