FIG. 6.

The effect of plakoglobin mutants on the localization of endogenous β-catenin. 293-T cells were transiently transfected with the HA-tagged plakoglobin constructs described in Fig. 1 and doubly stained for the HA tag (A, C, E, and G) and the endogenous β-catenin (B, D, F, and H). Cells were transfected with PG (A and B), PG ARM (C and D), CNX-PG ARM (E and F), and PG 1-ins (G and H). Note that PG ARM is efficient in translocating the endogenous β-catenin into the nucleus while PG 1-ins is not. The localization of the transfected proteins to cell-cell contacts is marked by arrows, while localization of CNX-PG ARM to cytoplasmic vesicles is marked by an arrowhead. Bar, 10 μm.