Fig. 6.

Secreted AFRUMA colocalizes with BiP in B3glct mutants, but UPR not activated. (A–C) Quantitation of corrected total cell fluorescence (CTCF) for AFRUMA (A) or BiP (B), and correlation coefficient for BiP and AFRUMA (C) in the intracellular and presecretory regions of the subcommissural organ and central canal (secreted). Representative images are shown in Figure 5. Fluorescence was measured from 14 sections located in the middle part of the subcommissural organ from five animals from each group (n = 5). Measurements for B3glct heterozygous controls (Δ/+) are plotted in blue dots; B3glct homozygotes (Δ/Δ) are plotted as red dots (mild ventriculomegaly) and black dots (severe hydrocephalus). (D) qRT-PCR analysis of UPR genes in subcommissural organ and brain cortex. Xbp1-T, -U and -S indicate total, unspliced and spliced form of Xbp1, respectively. Data were evaluated for statistical significance using unpaired, two-tailed t-test. ^*P ≤ 0.05, ^**P ≤ 0.01, ^***P ≤ 0.001, ns not significant.