Figure 5.

Immunolocalization of calpain1 in the retinas of nondiabetic (N) and 2-month diabetic mice (D). G–I: Sections from Capn1^−/− mice were used as negative control. A, D, and G: Control sections were treated with the serum from nonimmunized animals (NI). B, C, E, F, H, and I: Nuclei were stained with DAPI (in blue), and Capn1 (in red). All images are representative. n = 3 mice (approximately 5 months) and 1 retina from each mouse. Scale bars: 100 μm. GCL, ganglion cell layer; IPL, inner plexiform layer; IS, photoreceptors inner segment; ONL, outer nuclear layer; OPL, outer plexiform layer; OS, photoreceptors outer segment; WT, wild type.