Figure 9.

WWOX is involved in superoxide generation by 661W cells incubated in nondiabetic (5 mmol/L) and diabetic (30 mmol/L) glucose. A:Wwox gene expression was increased twofold in the diabetic-like glucose concentration, and calpain inhibitor inhibited diabetes-induced Wwox expression. 66W1 photoreceptors were incubated in low glucose (5 mmol/L = normal glucose) or high glucose (30 mmol/L = diabetes-like glucose concentration) without or with calpain inhibitor (10 μmol/L). mRNA levels were performed using quantitative RT-PCR. B and C:Wwox knockdown using siRNA inhibited the glucose-induced increase Wwox gene expression (B) and superoxide generation (C) as compared with cells treated with scrambled-siRNA. Data are expressed as means ± SD. n = 2 replications of the results . ∗∗P ≤ 0.01, ∗∗∗P ≤ 0.001.