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. 2021 Nov;191(11):2009–2022. doi: 10.1016/j.ajpath.2021.07.006

Supplemental Figure S1.

Supplemental Figure S1

Evidence of the successful transduction and knockdown of HL60 cells with Scramble (SCR) vector and mirZIP-378-3p (ZIP). A: Expression of the antisense to miR-378-3p by the lentiviral vector by quantitative real-time RT-PCR (RT-qPCR) showing expression of the antisense only the ZIP cells. B: Expression of miR-378-3p by RT-qPCR showing knockdown of the miRNA expression in ZIP cells. C: Expression of green fluorescent protein (GFP) by PCR and gel electrophoresis, showing successful transduction of the vector in SCR and ZIP cells. D: Fluorescent images cells demonstrating expression of GFP by SCR and ZIP cells. Original magnification, ×10 (D). UNT, untransduced.