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. 2021 Nov;191(11):2009–2022. doi: 10.1016/j.ajpath.2021.07.006

Figure 4.

Figure 4

Heatmaps of relative use of intracellular signaling pathways by CyTOF mass cytometry. Expression levels are normalized to the unstimulated paired condition, and the difference between two test arms after normalization represented on the heat map scale. The myeloid cells are binned into three stages of maturation (immature, intermediate, and mature). A and B: miRZip-378a-3p (ZIP) expression levels shown relative to either untransduced (UNT) or scramble (SCR) cells with all-trans retinoic acid (ATRA) (A) or ATRA plus azacytidine (B). Green indicates lower expression in the ZIP cells, whereas red indicates higher expression in the ZIP cells. C and D: Expression levels from samples of patients with myelodysplasia compared with control samples treated with dimethyl sulfoxide vehicle control (C) or azacytidine (D). Green indicates lower expression in the patient cells, whereas red indicates higher expression in the patient cells compared with controls. ∗P < 0.05. Casp-3, caspase-3; CREB, cAMP response element-binding protein; c-PARP, cleaved poly (ADP-ribose) polymerase; MAPK, mitogen-activated protein kinase; MDS, myelodysplastic syndromes; p-, phospho; PLCγ1, phospholipase C γ1.