Figure 5.

Azacytidine reverses the effects of miRZip-378a-3p (ZIP) on HL60 maturational morphologic features. ZIP and scramble (SCR) cells were assessed in presence of all-trans retinoic acid (ATRA) with dimethyl sulfoxide vehicle control or azacytidine. A–F: Morphologic assessment was performed on cytospins on SCR (A, C, and E) and ZIP cells (B, D, and F) at day 0 (D0) (A and B) and day 7 (D7) of ATRA (C and D) or ATRA and azacytidine (E and F). C and D: The untreated SCR cells demonstrate increased mature forms (C), whereas the ZIP cells show more intermediate forms (D). E and F: With ATRA treatment, there is an increase in the number of mature myeloid forms in the ZIP cells (F), such that there is no significant difference between SCR and ZIP (E versus F). Original magnification, ×40 (Wright-Giemsa stain).