Table 2.
List of CyTOF Mass Cytometry Antibodies, Clone, and Isotope Label
| Target | Clone catalog number | Isotope |
|---|---|---|
| Cell surface markers | ||
| CD3 | UCHT1 | 115In |
| CD45 | HI30 | 141Pr |
| CD11b | M1/70 | 142Nd |
| CD34 | 581 | 143Nd |
| CD235a | HI264 | 144Nd |
| CD16 | 3G8 | 145Nd |
| CD8a | RPA T8 | 146Nd |
| CD13 | WM15 | 147Sm |
| CD20 | 2H7 | 148Nd |
| CD123 | 6H6 | 151Eu |
| CD14 | M5E2 | 152Sm |
| CD33 | WM53 | 154Sm |
| CD4 | RPA T4 | 155Gd |
| CD56 | HCB6 | 162Dy |
| CD117 cKit | 10422 | 158Gd |
| CD274/PD-L1 | 29E.2A3 | 170Er |
| Intracellular markers | ||
| Akt | Poly6034 | 150Nd |
| c-PARP | F21-852 | 164Dy |
| Cleaved caspase 3 (Asp175) | D3E9 | 156Gd |
| Ki-67 | 8D5 | 161Dy |
| Phospho-JAK2 (Y1008) | D4D8 | 153Eu |
| Phospho–NF-κB p65 (Ser536) | 93H1 | 159Tb |
| Phospho-STAT1 (Y701) | 58D6 | 160Gd |
| Phospho-PLCγ1 (Tyr783) | D6M9S | 165Ho |
| Phospho-p38 MAPK (Thr180/Tyr182) | D3F9 | 166Er |
| Phospho-p44/42 MAPK (T202/Y204)/Erk1/2 | D13.14.4E | 167Er |
| Phospho–cleaved CBL (Tyr700) | D1D67 | 169Tm |
| Phospho-STAT4 (Y693) | D2E4 | 171Yb |
| Phospho–4E-BP1 (T37/46) | 236B4 | 172Yb |
| Phospho-STAT5 (Y694) | D47E7 XP (R) | 174Yb |
| Phospho-S6 (p70RSK) S235/236 | D57.2.2E XP R | 175Lu |
| Phospho-CREB (S133) | 87G3 | 176Yb |
c-PARP, cleaved poly (ADP-ribose) polymerase; CREB, cAMP response element-binding protein; MAPK, mitogen-activated protein kinase; PLCγ1, phospholipase C γ1; STAT, signal transducer and activator of transcription.