Table 3.

Manual Differential of Morphologic Stages of Myeloid Maturation and Dysplasia in UNT, SCR, and ZIP Cells in Primary HSCs (Mean of 4 Replicate Experiments, with Goal of 100 Cell Counts per Experiment) and the HL60 Cell Line (Mean of 4 Replicate Experiments, with Goal of 200 Cell Counts per Experiment)

Morphologic feature (mean lobes)	UNT, %	SCR, %	ZIP, %	P value
				SCR versus ZIP	UNT versus ZIP	UNT versus SCR
HSCs
Neutrophils (3–5 lobes)	30.50	7.00	0.00	0.0500	0.0001	0.0009
Neutrophils (2 lobes)	6.50	5.25	0.50	0.0500	0.0267	0.4918
Neutrophils (1 lobe)	0.25	12.00	5.75	0.0154	0.0034	0.0026
Intermediate myeloid	34.25	23.75	9.25	0.0344	0.0099	0.2209
Immature myeloid	25.25	13.75	3.00	0.0481	0.0036	0.0904
Apoptotic body	3.25	38.25	81.50	0.0021	<0.0001	0.0033
HL60s
Mature myeloid without significant atypia	32.12	26.25	4.41	0.0290	0.0027	0.5951
Mature myeloid and dysplastic	8.68	4.50	1.13	0.2319	0.0796	0.2599
Intermediate myeloid without significant atypia	32.07	28.75	53.92	0.0255	0.0450	0.7353
Intermediate myeloid and dysplastic	2.67	1.00	1.68	0.5664	0.7692	0.3198
Immature myeloid without significant atypia	12.22	29.25	27.36	0.8691	0.0148	0.3353
Immature myeloid and dysplastic	0.67	0.50	2.59	0.2129	0.3133	0.9929
Apoptotic body	11.56	9.75	8.92	0.7429	0.4120	0.1961
HL60s, azacytidine treated
Mature myeloid without significant atypia	36.79	34.92	26.59	0.7848	0.0628	0.2641
Mature myeloid and dysplastic	6.68	8.54	1.80	0.2722	0.1013	0.5838
Intermediate myeloid without significant atypia	39.25	21.83	35.58	0.4003	0.6572	0.0100
Intermediate myeloid and dysplastic	3.71	0.13	1.14	0.0622	0.1305	0.0263
Immature myeloid without significant atypia	5.84	19.27	22.01	0.8391	0.0619	0.2413
Immature myeloid and dysplastic	0.39	1.02	0.88	0.8811	0.5935	0.9601
Apoptotic body	7.33	14.29	12.01	0.7937	0.2318	0.4792

HSCs, human hematopoietic stem cells; SCR, scramble; UNT, untransduced; ZIP, miRZIP-378-3p.