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. 2021 Aug 14;62(8):1311–1320. doi: 10.1093/pcp/pcab095

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© The Author(s) 2021. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Fig. 6 — Effects of mutations to the EBS–IBS pairings and to δ′ (U) on the splicing of the pre-mRNA substrate. (A) Pairings between wild-type EBS1 and IBS1 (top) and wild-type EBS2 and IBS2 (bottom). (B) EBS1 mutated to mEBS1 and IBS1 mutated to mIBS1 (red letters indicate mutated nucleotides). (C) EBS2 mutated to mEBS2 (left), deletion of EBS2 to dEBS2 (middle, red bars indicate deleted nucleotides) and IBS2 mutated to mIBS2 (right). (D) Gel patterns of spliced products from atpF pre-mRNA. Lane 1: pre-mRNA, lane 2: synthetic spliced mRNA, lane 3: wild-type pairing, lane 4: mEBS1–IBS2 pairing, lane 5: EBS1–mIBS1 pairing, lane 6: mEBS2–IBS2 pairing, lane 7: dEBS2–IBS2 pairing, lane 8: EBS2–mIBS2 pairing, lane 9: U to a (red), lane 10: U to c (red), and lane 11: U to g (red) at the δ′ nucleotide (U). M: size markers.