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. 2021 Sep 27;18(16):3728–3737. doi: 10.7150/ijms.64253

Figure 2.

Figure 2

CEBPA-DT downexpression enhances cisplatin sensitivity and facilitates cell apoptosis in cisplatin-resistant OSCC cells. (A) qRT-PCR detected the transfection efficiency of CEBPA-DT smart silencer (ss-CEBPA-DT) and negative control (ss-NC) in Cal27-CisR and HSC4-CisR cells. (B) CCK-8 assay detected the IC50 value decrease in ss-CEBPA-DT transfected chemoresistance cells compared with ss-NC transfected group. (C) qRT-PCR detected CEBPA-DT overexpression (pc-CEBPA-DT) and corresponding control (pc-NC) transfection efficiency in Cal27-CisR and HSC4-CisR cells. (D) CCK-8 assay show IC50 increase in CEBPA-DT overexpression (pc-CEBPA-DT transfected) chemo-resistance cells compared with control (pc-NC transfected) group. (E-F) Cell apoptosis rate was detected in ss-CEBPA-DT and ss-NC transfected chemo-resistance cells through Annexin-V-FITC & PI staining assay (E) and TUNEL staining assay (F). N=3~5 independent experiments, *P < 0.05.