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. 2021 Oct 15;18(16):3768–3779. doi: 10.7150/ijms.58415

Figure 2.

Figure 2

NSE promoted the proliferation, migration and invasion of SCLC cells. The relative mRNA expression levels of NSE in human bronchial epithelial cell 16HBE and various SCLC cell lines were detected using real time quantitative PCR (A). (B) The relative protein express levels of NSE were measured by western blot. And quantification of the relative protein amount was shown in the lower panel. (C) Real time quantitative PCR confirmed the expression of NSE after being overexpressed or knockdown. (D) The effect of NSE on the proliferation capabilities of SCLC cells were assessed using CCK8 assay. Transwell assay was performed to investigate the effect of NSE on the migration and invasion of SCLC cells. (E) Overexpression of NSE promoted cell migration and invasion of H446 cells. (F) Knockdown of NSE significantly inhibited cell migration. (G) Western blot results demonstrated overexpression of NSE promoted the EMT process by down-regulating the expression of E-cadherin and upregulating the expression of N-cadherin, while silencing NSE upregulated the expression of E-cadherin and downregulated the expression of N-cadherin. And quantification of the relative protein amount was shown in the lower panel.