Figure 5.

Artesunate inhibited the translocation of nuclear factor (NF)-κB. (a) Brain sections were stained with anti‐NF-κB (red) and Hoechst (blue) to label NF-κB and the nucleus, respectively. The merged area indicates active transcription. (b) Quantification of cells labeled with anti-NF-κB in the nucleus. (c) Western blotting analysis and quantification of the nuclear NF-κB protein level in the cerebral cortex in different groups at 72 hours after distal middle cerebral artery occlusion (dMCAO). (d) Western blotting analysis and quantification of the protein levels of cytosolic inhibitor protein alpha (Iκ-B) in different groups at 72 hours after dMCAO (*P < 0.05 vs the dMCAO group, **P < 0.001 vs the dMCAO group).