Figure 1.

Isolation, identification and labeling of donor cells from sexually mature male turbot. (A1) The survival rate of cells in the testes after whole cryopreservation were determined by trypan blue staining. (A1') Histology of germ cells distributed at the edge of the testes. (A2-A5) Four distinct cell bands were generated in the tube after Percoll density gradient centrifugation. (B) The qPCR was used to detect the expression of oct4, nanos2, plzf and vasa genes in isolated cells. (C) The first cell band was stained with PKH26 and DAPI. The asterisk in A1 indicated dead cell. Aund: type A undifferentiated spermatogonia; Adiff: type A differentiated spermatogonia; SpgB: type B spermatogonia. Different letters indicated statistically significant differences (P < 0.05). The insets were magnification of one spermatogonia. Scale bar, 15 µm.