Figure 6.

Schematic illustration of spermatogonial stem cell transplantation whin Pleuronectiformes. The spermatogonia of Japanese flounder, summer flounder, and turbot were isolated from cryopreserved whole testes, then labeled and transplanted into the peritoneal cavities of triploid Japanese flounder larvae. The transplanted recipients differentiated into male and female chimeras in intra-species and intra-genus transplantations, and only male and intersex chimeras in inter-family transplantations. Finally, donor-derived spermatozoa and the offspring by artificial insemination were obtained from recipients at 2 ypt, respectively.