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. 2021 Oct 11;17(15):4165–4175. doi: 10.7150/ijbs.63889

Figure 2.

Figure 2

SOCS2 suppresses inflammation via limiting the activation of NF-κB signaling pathway. A. The related protein and mRNA level of SOCS2 was detected by Western blot and RT-PCR in RAW cell lines overexpreesing SOCS2 (RAW-Ctrl/ SOCS2+). B. The related protein and mRNA level of SOCS2 was detected by Western blot and RT-PCR in RAW cell lines knockdown SOCS2 (RAW-Ctrl/ shSOCS2). C. The related mRNA level of TNFα, IL-1β, IL-6, IL-8, IL-17, IL-18 was detected by RT-PCR in RAW-Ctrl or -SOCS2+ or -shSOCS2 treated with FFAs for 24 hr. D. The level of TNFα, IL-1β, IL-6, IL-8, IL-17, IL-18 was detected by ELISA in RAW-Ctrl or -SOCS2+ or -shSOCS2 treated with FFAs for 24 hr. E. Immunoblotting of phospho-IKKα, IKKα, phospho-IKKβ, IKKβ, phospho-P65, P65, SOCS2, and β-actin (loading control) in RAW-Ctrl or -SOCS2+ or -shSOCS2 treated with FFAs for 24 hr. F. The levels of IL-1β, TNFα, IL-6, IL-8, IL-17, and IL-18 in cell culture supernatants from RAW-Ctrl and RAW-shSOCS2 cells treated with or without FFAs and/or PDTC for 24 hr were detected by ELISA.