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. 2021 Oct 11;17(15):4165–4175. doi: 10.7150/ijbs.63889

Figure 3.

Figure 3

SOCS2 in macrophages suppresses inflammation via limiting the activation of inflammasomes signaling pathway. A. Immunoblotting of NLRP3, Pro-Caspase-1, Caspase-1 p20/p22 and β-actin (loading control) in RAW-Ctrl or -SOCS2+ or -shSOCS2 treated with FFAs for 24 hr. B. The levels of IL-1β, TNFα, IL-6, IL-8, IL-17, and IL-18 in cell culture supernatants from RAW-Ctrl and RAW-shSOCS2 cells treated with or without FFAs and/or MCC950 for 24 hr were detected by ELISA. C. The related mRNA level of TNFα, IL-1β, IL-6, IL-8, IL-17, IL-18 was detected by RT-PCR in RAW-Ctrl and RAW-shSOCS2 cells treated with or without FFAs and/or MCC950 for 24 hr were detected by ELISA.