Figure 6.
Tandem CAR-T cells mediate a potent and lasting antitumor activity in xenografts. The treatment scheme is shown in (A). B-NDG mice were injected subcutaneously (s.c.) with 5 ×106 SNU119-Luc cells on day -10 and intravenously (i.v.) treated with 1 ×107 Control, MSLN-CAR, FOLR1-CAR, or Tandem CAR-T cells on day 0. (B) BLI of mouse tumor burdens at the indicated time points, representative of all experiments (n = 5 mice per group). (C) Mouse tumor burdens (average radiance) are presented. (D) Survival analyses comparing mice treated with Tandem CAR-T cells with those treated with Control, MSLN-CAR, or FOLR1-CAR T cells. P values were calculated with a one-sided log-rank Mantel-Cox test. (E) Tumor volume was analyzed with calipers. Data are shown as the mean ± SD (n=5). *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001.