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Inhibition of apoptotic ratio by P-PostC (propofol-Postconditioning) at the concentration 25 µmol/L in H9c2 cells subjected to H/R under hyperglycemia. a Apoptotic cells marked by Hoechst 33,258 staining (200 ×). b Counts of apoptotic cells by flow cytometry. c Mean Hoechst 33,258 fluorescence positive intensity. d Quantification of apoptotic ratio. Data are mean ± SEM from three independent experiments each performed in triplicate. *P < 0.05, **P < 0.01
