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NR treatment and quantification of acetylated histones. A, B Relative ATP, NAD+, NADH, and NAD+/NADH levels in NR treatment SY5Y (A) and U251 (B) cells, NR: cells treatment with NR, Control: cells without NR treatment. C, D H3K27Ac levels to the transcription start site (TSS) of NMNAT2 in SH-SY5Y (C) and U251 (D) cells. Data are shown as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, student’s t-test
