TABLE 2.
Plasmids used
| Plasmid | Characteristic(s) | Source |
|---|---|---|
| pEG202+PL | 2μm HIS3 ADH1p-LexA(DBD)-MCS-ADH1t | 33 |
| pJG4-5 | 2μm TRP1 GAL1p-B42(TAD)-MCS-ADH1t | 33 |
| pRS314 | CEN ARS TRP1 | 73 |
| pRS424 | 2μm TRP1 | 73 |
| pRS316 | CEN ARS URA3 | 73 |
| YCplac22 | CEN ARS TRP1 | 30 |
| YEplac112 | 2 μm TRP1 | 30 |
| pEMBLyex4 | 2 μm leu2d URA3 GAL1,10(UAS)-CYC1 | 17 |
| YEp352 | 2 μm URA3 | 37 |
| YEp351GAL | 2 μm LEU2 GAL1,10p | 8 |
| pASZ11 | CEN ARS ADE2 | 77 |
| pGEX4T3 | AmprT7-GST-MCS | Pharmacia |
| pET9d-His6-TEV | KanrT7-His6-TEV-MCS | G. Stier |
| pET8c | AmprT7-His6-MCS | S. Labeit |
| pRSETA | AmprT7-His6-MCS | Invitrogen |
| pTrcHisA | AmprTrc-His6-MCS | Invitrogen |
| pGALPATG | CEN ARS LEU2 GAL1p-ProtA-TEV-GAL4t | This study |
| pNOPPATA-TRP1-GSP2 | CEN ARS TRP1 NOP1p-ProtA-TEV-GSP2-ADH1t | This study |
| pEG-KG | 2μm leu2d URA3 GAL1,10(UAS)-CYC1-HA | I. Macara |
| pASZ11-xpo1-1 | H. Santos-Rosa | |
| pRS316-XPO1 | This study | |
| pMK330 | pRS316-CSE1 | 46 |
| pMK103 | YEp352-YRB1 | Künzler et al., submitted |
| pEMBLyex4-NUP2 | This study | |
| pEG-p36 | pEG-KG-YRB2 | I. Macara |
| pGEX-GSP1 | pGEX4T3-GSP1 | B. Senger |
| pLDB450-wt | pTrcHisA-GSP1 | This work |
| pMK104 | pRSETA-YRB1 | Künzler et al., submitted |
| pMK263 | pRSETA-RNA1 | This study |
| pGEX-YRB1 | pGEX4T3-YRB1(1-201) | This study |
| pGEX-YRB1-51 | pGEX4T3-yrb1-51(1-201) | This study |
| pGEX-YRB1-52 | pGEX4T3-yrb1-52(1-201) | This study |
| pGEX-YRB1RBD | pGEX4T3-YRB1(59-201) | This study |
| pGEX-YRB1N | pGEX4T3-YRB1(3-58) | This study |
| pGEX-YRB1RBDN | pGEX4T3-YRB1(59-131) | This study |
| pGEX-YRB1RBDC | pGEX4T3-YRB1(132-201) | This study |
| pGEX4T3-yrb1Δ(102-120) | This study | |
| pGEX4T3-yrb1-Δ(121-130) | This study | |
| pET9d-His6-TEV-XPO1 | This study | |
| pET9d-His6-TEV-CSE1 | This study | |
| pET8c-MTR10 | B. Senger | |
| pNOPPATA-TRP1-XPO1 | This study | |
| pNOPPATA-TRP1-CSE1 | This study | |
| pGALPATG-XPO1 | This study | |
| pMK275 | pRS314-YRB1 | Künzler et al., submitted |
| pMK277 | pRS314-yrb1-51 | Künzler et al., submitted |
| pMK278 | pRS314-yrb1-52 | Künzler et al., submitted |
| pRS314-yrb1-Δ(167-201) | This study | |
| pRS314-yrb1-Δ(132-201) | This study | |
| pRS314-yrb1-Δ(3-58) | This study | |
| pRS314-yrb1-Δ(59-131) | This study | |
| pRS314-yrb1-Δ(59-101) | This study | |
| pRS314-yrb1-Δ(102-131) | This study | |
| pRS314-yrb1-Δ(102-120) | This study | |
| pRS314-yrb1-Δ(121-130) | This study | |
| pRS314-yrb1-Δ(102-111) | This study | |
| pMK385-wt | pRS314-NLSSV40-YRB1 | This study |
| pMK385-51 | pRS314-NLSSV40-yrb1-51 | This study |
| pMK385-52 | pRS314-NLSSV40-yrb1-52 | This study |
| pRS314-NLSSV40-yrb1-Δ(121-131) | This study | |
| pMK284n | pRS314-YRB1-GFP(S65T) | 36 |
| pMK286n | pRS314-SRP1-GFP(S65T) | 46 |
| pMK346STOP-wt | YEp351GAL-muRanBP1-GFP(S65T) | This study |
| pMK346STOP-ΔC | YEp351GAL-muRanBP1ΔC-GFP(S65T) | This study |
| pMK291-wt | pRS424-YRB1-GFP(S65T) | Künzler et al., submitted |
| pMK291-51 | pRS424-yrb1-51-GFP(S65T) | Künzler et al., submitted |
| pMK291-52 | pRS424-yrb1-52-GFP(S65T) | Künzler et al., submitted |
| pRS424-yrb1-Δ(167-201)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(132-201)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(3-58)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(59-131)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(59-101)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(102-131)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(102-120)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(121-131)-GFP(S65T) | This study | |
| pRS424-yrb1-Δ(102-111)-GFP(S65T) | This study | |
| pRS424-YRB1-3xGFP(S65T) | This study | |
| pMK379-wt-n | pRS424-NLSSV40-YRB1-GFP(S65T) | This study |
| pMK379-51-n | pRS424-NLSSV40-yrb1-51-GFP(S65T) | This study |
| pMK379-52-n | pRS424-NLSSV40-yrb1-52-GFP(S65T) | This study |
| pRS424-NLSSV40-yrb1-Δ(121-131)-GFP(S65T) | This study | |
| pMK375 | YEplac112-YAP1-sGFP | This study |
| pEG202+PL-XPO1 | 54 | |
| pEG202+PL-MTR10 | 54 | |
| pEG202+PL-CSE1 | This study | |
| pJG4-5-Rev | 54 | |
| pJG4-5-GSP1 | This study | |
| pJG4-5-YRB1 | This study | |
| pJG4-5-SRP1 | This study |