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The effect of 7×2b CAR-T cells on tumor cells. (A) The expression of GD2 in melanoma and neuroblastoma cell lines. (B) After co-incubation for 12 h at a ratio of 10:1, the supernatant was removed to detect the IFN-γ secretion level by ELISA. (C) Luciferase-based reporter assays were used to detect the lysis (%) of 7×2b CAR-T cells against three melanoma and neuroblastoma cells with different E:T ratios.
