FIG 3.

Genetically conserved 9-mer peptides with highly networked amino acid residues and restricted to HLA-A*02:01 allele. Network scores (blue bars), predicted percent bind level to the HLA class I molecule (pink bars) and percent genetic variability across SARS-CoV-2 variants (gray bars) within the peptide repertoires derived from SARS-CoV-2 NC N-terminal RNA binding domain (a), the NC C-terminal dimerization domain (b), and the S protein (c) are shown. For the S protein, the 9-mers with the network score of at least 100 and the bind level of at least 95% are shown among the repertoire of 1112 peptides. All the network scores presented in this figure are normalized. The thresholds for network scores (blue line) are determined by the lower 95% confidence of the mean. The cutoffs for the percent bind level (red line) equates to the 95th percentile. The peptides with the network scores and percent bind level above the cutoffs and those which are conserved (i.e., 0% genetic variability) across the SARS-CoV-2 isolates are highlighted in red.