FIG. 7.

IGF-1 rescue of c-Myc-induced apoptosis is mutation specific. (A) Flow cytometry of wt Myc and MycS62A reconstituted cells cultured for 18 h in 0.1% serum in the presence or absence of IGF-1 (200 ng/ml). Both floating and attached cells were harvested, fixed, and stained with propidium iodide before determination of the DNA content by flow cytometry. (B) Apoptotic activity of different c-Myc mutants after gating for the sub-G₁ population in serum-starved cells with or without IGF-1. Data represent the averages of three independent experiments ± standard error.