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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Autism Res. 2020 Oct 27;14(1):29–45. doi: 10.1002/aur.2418

Figure 4.

Figure 4.

Subcutaneous injection of IGF-2 or M6P reverses motor deficits and marble burying impairment of Ube3am−/p+ mice. (A–C) Experimental schedules are shown above graphs. S.c. injection of IGF-2, M6P, or respective vehicles (↑) was administered 20 min before commencement of behavior. (A) Hindlimb clasping behavior. Log transformation of hindlimb clasping scores of WT and Ube3am−/p+ mice. (B) Accelerating rotarod. Latency in seconds of falling off a rotating rod in WT and Ube3am−/p+ mice tested 20 min, 48 hr, and 7 days following injection. (C) Marble burying. Number of marbles buried, and time spent burying marbles (in seconds) by WT and Ube3am−/p+ mice 20 min after injection. All data are expressed as means ± s.e.m.; n = 6–14 per group. Two-way analysis of variance (ANOVA) followed by Tukey’s post-hoc tests. *P < 0.05, **P < 0.01, ***P < 0.001. n.s., not significantly different.