Table 1.

A s.c. IGF-2 or M6P Injection Does Not Produce Adverse Effects in Either WT or Ube3a^m−/p+ mice

	30-min test
	WT Veh (n = 8)	AS Veh (n = 7)	WT IGF-2 (n = 6)	AS IGF-2 (n = 6)	WT M6P (n = 6)	AS M6P (n = 6)
Physical characteristics
Weight (males)	25.0 ± 0.55	29.0 ± 0.58	25.3 ± 0.33	29.3 ± 0.81	25.3 ± 0.88	29.0 ± 0.58
Weight (females)	19.3 ± 0.67	22.0 ± 1.15	20.7 ± 0.81	22.3 ± 0.33	21.3 ± 0.88	22.7 ± 0.33
Temp	36.7 ± 0.05	36.7 ± 0.03	36.8 ± 0.05	36.7 ± 0.03	36.8 ± 0.06	36.7 ± 0.03
Whiskers (% with)	100	100	100	100	100	100
Bald patches (% with)	0	0	0	0	0	0
Palpebral closure (% with)	0	0	0	0	0	0
Exothalmos (% with)	0	0	0	0	0	0
General behavioral observations (% of subject displaying response)
Wild running	0	0	0	0	0	0
Freezing	0	0	0	0	0	0
Sniffing	100	100	100	100	100	100
Licking	0	0	0	0	0	0
Rearing	100	100	100	100	100	100
Jumping	0	0	0	0	0	0
Defecation	25	29	33	33	17	33
Urination	0	0	0	0	17	0
Sensorimotor reflexes (% of subjects showing normal response)
Righting	100	100	100	100	100	100
Whiskers	100	100	100	100	100	100
Eye-blink	100	100	100	100	100	100
Ear-twitch	100	100	100	100	100	100
	24-hr test
Physical characteristics
Weight (males)	25.6 ± 0.75	29.2 ± 0.48	26.3 ± 0.88	29.7 ± 1.1	26.7 ± 1.3	29.7 ± 0.33
Weight (females)	20.0 ± 0.58	22.0 ± 1.15	21.3 ± 0.82	22.7 ± 0.67	21.0 ± 0.58	22.7 ± 0.33
Temp	36.7 ± 0.05	36.7 ± 0.03	36.8 ± 0.04	36.8 ± 0.07	36.7 ± 0.03	36.7 ± 0.03
Whiskers (% with)	100	100	100	100	100	100
Bald patches (% with)	0	0	0	0	0	0
Palpebral closure (% with)	0	0	0	0	0	0
Exothalmos (% with)	0	0	0	0	0	0
General behavioral observations (% of subject displaying response)
Wild running	0	0	0	0	0	0
Freezing	0	0	0	0	0	0
Sniffing	100	100	100	100	100	100
Licking	0	0	0	0	0	0
Rearing	100	100	100	100	100	100
Jumping	0	0	0	0	0	0
Defecation	0	0	17	0	17	33
Urination	0	14	0	0	0	0
Sensorimotor reflexes (% of subjects showing normal response)
Righting	100	100	100	100	100	100
Whiskers	100	100	100	100	100	100
Eye-blink	100	100	100	100	100	100
Ear-twitch	100	100	100	100	100	100
	7-day test
Physical characteristics
Weight (males)	25.6 ± 0.75	29.2 ± 0.48	26.3 ± 0.88	29.3 ± 1.08	27.3 ± 0.67	29.7 ± 0.33
Weight (females)	20.0 ± 0.85	22.7 ± 1.2	21.7 ± 0.82	23.0 ± 0.58	21.3 ± 0.88	23.0 ± 0
Temp	36.8± 0.04	36.7 ± 0.02	36.7 ± 0.04	36.7 ± 0.02	36.7 ± 0.03	36.7 ± 0.1
Whiskers (% with)	100	100	100	100	100	100
Bald patches (% with)	0	0	0	0	0	0
Palpebral closure (% with)	0	0	0	0	0	0
Exothalmos (% with)	0	0	0	0	0	0
General behavioral observations (% of subject displaying response)
Wild running	0	0	0	0	0	0
Freezing	0	0	0	0	0	0
Sniffing	100	100	100	100	100	100
Licking	0	0	0	0	0	0
Rearing	100	100	100	100	100	100
Jumping	0	0	0	0	0	0
Defecation	13	14	33	33	33	17
Urination	0	0	0	0	0	0
Sensorimotor reflexes (% of subjects showing normal response)
Righting	100	100	100	100	100	100
Whiskers	100	100	100	100	100	100
Eye-blink	100	100	100	100	100	100
Ear-twitch	100	100	100	100	100	100

Observational battery of general physical characteristics and motor and sensory responses of WT and Ube3a^m−/p+ (AS) mice injected with IGF-2, M6P, or Vehicle (Veh) tested 30 min, 24 hr and 7 days after injection.