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. 2021 Nov 10;9(3):e01661-21. doi: 10.1128/Spectrum.01661-21

FIG 4.

FIG 4

CDK1 inhibits the MAVS-mediated IFN-β induction by increasing the miR-22 expression. (A to C) HeLa cells were transfected with 3×FLAG-CMV-10-CDK1 or an empty vector for 24 h. Samples were collected and were processed for Western blotting to detect the phosphorylation status of CDK1 protein (A) and for qRT-PCR analysis to determine the mRNA levels of miR-22 (B) and MAVS (C). (D) HeLa cells were transfected with 3×FLAG-CMV-10-CDK1 or an empty vector for 24 h. Subsequently, cells were subjected to poly(I·C) treatment for a period of 12 h, and then the mRNA levels of IFN-β were measured by qRT-PCR. (E) HeLa cells were cotransfected with 3×FLAG-CMV-10-CDK1 or empty vector and miR-22 inhibitor or negative-control (NC) inhibitor (final concentration, 100 nM) for 24 h, followed by treatment of cells with poly(I·C) for another 12 h. qRT-PCR was performed to analyze the mRNA levels of MAVS. All data were pooled from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001. ns, nonsignificant.